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10% w/v, pH 7.4) using Omni-125 hand-held homogenizer (Omni International, USA), then the homogenates were centrifuged using a cooling centrifuge at (2000 g , 15 min, 4 C), and the supernatant was divided into aliquots, where one aliquot was freshly used for assay of oxidative biomarkers, and the other one stored at 80 C to be used for enzyme-linked immunosorbent assay (ELISA)

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