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Pathological evidence indicates that AD is also associated with degeneration in cholinergic neuron-rich regions, such as the nucleus basalis of Meynert, frontal cortex, and anterior and posterior cingulate cortex, which can lead to the symptoms of memory impairment and agitation

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An EIA-GLP-1 ELISA kit (RayBio, Norcross, GA) was used to detect GLP-1 released into the assay medium

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