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its research value is more mechanistic (studying isolated lipolysis) than outcome-driven
While primers sequence for IL8, HSP27 and SOD1 were designed using Primer3 ( and Primer-BLAST ( Primer specificity was verified by running PCR for 40 cycles at 95 C for 10 seconds and 60 C for 60 seconds, followed by gel electrophoresis on a 3% agarose gel stained with SYBR safe DNA gel stain
Equipment quality affects contamination rates significantly
Six intact SD rats were used for urinary, fecal, and biliary excretion studies
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