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The study of spontaneous metastasis is hindered since the required interval for spontaneous metastasis to occur in vivo takes much longer than the commonly used intravenous inoculation of tumor cells, in addition animal wellness rules in almost all institutions, frequently discourages the practice to keeping tumor-bearing mice for a long period of time
Interestingly, the clinical manifestations of the disorder can be improved by supplementation of high doses of carnitine
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When GHK encounters Cu under physiological conditions, the histidine imidazole nitrogen and the N-terminal amine coordinate the metal, with lysine contributing to the binding pocket
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