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Total protein concentrations were determined using the bicinchoninic acid (BCA) method by measuring absorbance at 562 nm

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Glutathione-S-transferase (GSTP) that participates in the conjugation of GSH to reactive cysteines (S-glutathionylation) seems to play an important role in idiopathic pulmonary fibrosis lung fibrogenesis, since GSTP immunoreactivity is increased in the lungs of idiopathic pulmonary fibrosis patients, notably within type II epithelial cells (128, 129)