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This multicenter study is enrolling 17,500 adults aged 45 or older with a body mass index (BMI) of 27 kg/m2 or greater and established CVD but without diabetes

Assessment of cell differentiation The differentiated C2C12 cells were stained with Crystal Violet Staining Solution (Beyotime, China), and images were taken using an inverted microscope (Nikon ECLIPSE Ti, Japan)

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Chapela SP, Martinuzzi ALN, Llobera ND, Ceriani F, Gonzalez V, Montalvan M, et al