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DIA MS2 scans were acquired in the Astral mass analyser with a 3 ms maximum injection time using a variable windowing scheme, using 2 Da windows from 380 to 680 m/z , 4 Da windows from 680 to 800 m/z and 8 Da windows from 800 to 980 m/z
Peptides break down in the gut, so the sublingual route is preferred for consistent systemic delivery without the need for injection
Laviano, A., Molfino, A., Lacaria, M
The concentration of Mouse GMCSF in the samples is then determined by comparing the OD of the samples to the standard curve
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