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For coculture experiments, 2 million THP-1 cells were seeded in each well of a fresh 12-well plate and differentiated with PMA (100 nM) following a 24-hour rest period
however, this approach is subject to much stricter criteria and may involve a long waiting list
This challenge is partly due to the increasing obesity prevalence and its impact on T2DM as well as T1DM pathophysiology and prognosis [15,16,17,18,19,20,21]
Gastrointestinal side effects, including constipation, nausea, and altered bowel habits, occur frequently with these medicines
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