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The filtrate was concentrated by centrifugation, and metabolites corresponding to 5 10 cells were dissolved in 5 L of ammonium hydroxide before being analyzed by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS)

Animals tests for acute skin irritation assessment usually follow the Draize rabbit test while other accepted assays for skin sensitization include Local Lymph Node Assay (LLNA) and Guinea Pig Maximisation Test (GPMT)

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Insulin allows glucose to enter the cells and be converted into energy