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The reconstituted peptide is then stored according to its own protocol, while the bacteriostatic water vial can be returned to refrigeration for the next withdrawal
The cells were washed and resuspended in ice-cooled phosphate-buffered saline in centrifuge tubes
As a member of the GPCR family, ChemerinR1 has a typical structure of seven transmembrane -helices, and there are G protein binding sites on the C-terminal of its peptide chain and the intracellular loop connecting the fifth and sixth transmembrane helices (Kaur et al., 2010)
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