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Lawson, O
For the immunostaining of cultured PSCs, cells were fixed in 4% PFA for 15 min followed by a 15-min incubation in 0.5% Triton-X100
As gut microbiota are highly responsive to diet, and nutritional support is already a protocolized part of TBI care (due to observational studies demonstrating an association between delayed enteral nutrition and worse neurologic outcome [113]) there has been much interest in identifying dietary interventions which can improve recovery and reduce mortality [114]
Patients report feeling fundamentally different in their baseline energy, mental sharpness, physical resilience, and overall vitality
