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doi: 10.1016/j.cgh.2018.01.018 549 RaoSCAthalye-JapeGKDeshpandeGCSimmerKNPatoleSK

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Acetylation: NATs catalyze a ping-pong bi-bi mechanism: acetyl-CoA first acetylates a cysteine residue (Cys68) in the NAT active site, releasing CoA, and then the acetyl group is transferred from Cys68 to the substrate amine