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We utilized Superscript III reverse transcriptase enzyme (Invitrogen) with random hexamer primers and 3 g of RNA from each sample to produce cDNA according to the manufacturers protocol
(December 1980)
Our study was in line with the previous study that upregulation of FTH could alleviate cisplatin induced HK2 cell injury through anti-ferroptosis (Hu et al., 2020)
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