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Beads were collected and washed twice with IP buffer, twice with high-salt wash buffer (20 mM Tris-HCl, pH 8.0, 500 mM NaCl, 1% Triton X-100, 2 mM EDTA, 0.1% SDS, supplemented with the cOmplete proteinase inhibitors), twice with LiCl wash buffer (10 mM Tris-HCl, pH 8.0, 250 mM LiCl, 1% NP-40, 2 mM EDTA, supplemented with the cOmplete proteinase inhibitors), and once with cold TE+NaCl wash buffer (10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 50 mM NaCl)

Defense teams also attack causation, arguing that a patients high-fat diet, preexisting conditions, or genetics could explain the disease

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L-C19 patients developed endothelial dysfunction which, even if it had progressively improved after 6 months, the risk for thrombotic or cardiovascular events remained high