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Throughout both the noise exposure and the silent period, the animals were monitored every 15 min and returned to their cages afterward
Additionally, nanoparticles also address common issues associated with conventional ocular formulations, such as eye irritation and discomfort
The cDNA was then fourfold diluted and used as a template in quantitative PCR with IQ SYBR Green Supermix (Bio-Rad) on a CFX96 instrument (Bio-Rad) using a 3-step protocol
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