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Immunoblotting Before SDSPAGE analysis, samples were boiled in 2 Laemmli sample buffer (125 mM Tris-HCl, pH 6.8, 10% 2-mercaptoethanol, 4% SDS, 10% sucrose, 0.01% bromophenol blue (BPB)) for 5 min
BioTech Pharma website at Accessed March 5, 2026.
Figure S4
Additionally, it suppresses macrophage ferroptosis and reduces inflammation by upregulating Nrf2 expression in high-glucose-treated macrophages, collectively promoting the healing of full-thickness skin defect wounds in diabetic rats [76, 79]
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