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A total of 350 g of whole cell lysates from H69 cells with or without HGF stimulation was used for each KPSS phosphoantibody array screen, which is an antibody-based method (Kinetworks) that relies on sodium dodecyl sulphate (SDS)-polyacrylamide minigel electrophoresis and multilane immunoblotters to permit the specific and quantitative detection of numerous protein kinases (PK) or other signal transduction proteins simultaneously (Pelech et al, 2003)

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