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92 IkedaYAiharaKAkaikeMSatoTIshikawaKIseTet al
This fragment was digested with EcoRI (NEB) and ligated into pGex-6p1 (GE Healthcare) prepared by digestion with BamHI and blunting with Mung Bean Nuclease (NEB) to generate pJB-GST-HTS
However, the NF-B binds to inhibitor IB in an inactive phase that segregates the transcription factor in the cytoplasm
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