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Description

The cells were harvested by centrifugation at 1,500 g for 3 min at room temperature and subsequently resuspended in an isosmotic buffer (sorbitol 0.1 M, TrisHCl pH 7.4 0.1 M, NaCl 0.1 M) to a final concentration of 1.5 OD 600 units/ml (where 1 OD 600 unit represents 1 ml of culture with an OD 600 = 1)