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The off period is mainly there to address copper-accumulation concern from the GHK-Cu component, which makes up 62.5% of the blend
It differs a bit from one person to another, the effects of B12 vitamins usually last around 48 hours to 72 hours after the initial injection
Even refrigerator light (which cycles on and off with door opening) can contribute to photodegradation over weeks of repeated exposure
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